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How to Reconstitute Peptides (Step by Step)

6 min read|By James Quilter

Reconstituting peptides is straightforward once you understand the process. The goal is to dissolve the freeze-dried powder into bacteriostatic water so it can be accurately dosed and administered.

What you need: your peptide vial, bacteriostatic water, an insulin syringe, alcohol swabs, and a clean workspace. Sterile technique is important. Wash your hands and work on a clean surface.

Step 1: Let your peptide vial and bacteriostatic water come to room temperature. Do not use them straight from the freezer or refrigerator. This takes about 15 to 20 minutes.

Step 2: Swab the tops of both the peptide vial and the bacteriostatic water vial with alcohol wipes. Let them air dry.

Step 3: Draw your desired amount of bacteriostatic water into the syringe. How much water to add depends on your peptide amount and desired concentration. Use our Peptide Calculator to figure out the right amount.

Step 4: Insert the needle into the peptide vial at an angle and slowly let the water run down the side of the glass. Do not squirt it directly onto the powder. Tilt the vial at 45 degrees and let gravity do the work. This prevents foaming and preserves peptide integrity.

Step 5: Gently swirl the vial until the powder is fully dissolved. Do not shake it. If there are still particles, let it sit in the refrigerator for 15 to 30 minutes and swirl again.

Step 6: Store the reconstituted peptide in the refrigerator. It will remain stable for approximately 3 to 4 weeks. Never freeze a reconstituted peptide and avoid repeated temperature changes.

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